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KMID : 0881720170320030199
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Journal of Food Hygiene and Safety
2017 Volume.32 No. 3 p.199 ~ p.205
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Real-time PCR assay for the Detection of Toxoplasma gondii in Retail Meats: Proof-of-concept Study
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Yun Han-Seong
Suh Soo-Hwan
Kwak Hyo-Sun
Joo In-Sun
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Abstract
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Although many PCR-based assays have been developed, the majority of rapid detection of Toxoplasma gondii in animal and their meat product has been dependent on immunogenic assays. Thus, there is still a need for more reliable PCR based detection method for T. gondii in retail meats. Recently, a 529-bp repeat element that exists in 200-300 copies per genome of T. gondii genome had been spotlighted for its usefulness as potential detection targers. In this study, the 529-bp repeat element was selected for real-time PCR to detect three types of T. gondii (type I, II and III). A primer pair targeting 82-bp of the 529-bp element detected all three types of T. gondii and showed high level of specificity against 14 different food-borne pathogens as well as 3 protozoan parasites such as Giardia lamblia, Cryptosporidium parvum and Entamoeba histolytica. Application of the new real-time PCR assay in meat samples showed improved detection sensitivity compared to the B1-gene targeted method suggesting potential new target for Toxoplasma gondii screening in retail meats.
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KEYWORD
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Toxoplasma gondii , Real-time PCR , Retail meat
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